HSP27 Antibody

SKU: orb1822470

Description

Mouse Anti-Human HSP27 Monoclonal IgG2b Kappa

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Reactivity: Human
• Application Notes:
  0.5 µg/ml was sufficient for detection of HSP27 in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b Kappa
• Clone No.: 5D12-A12
• Immunogen: Full length human HSP27
• Target: HSP27
• Molecular Weight: 27kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSPB1, HSP27, HSP25, HSP28, Heat shock protein beta-1, Heat shock 27 kDa protein, MKBP, DMPK-binding protein, SRP27, 28kDa heat shock protein, CMT2F

Quick Database Links

Gene Symbol

HSP27

UniProt

P04792

NCBI Gene ID

3315

RefSeq (Protein):NP_001531.1