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Catalog Number | orb1822470 |
---|---|
Category | Antibodies |
Description | Mouse Anti-Human HSP27 Monoclonal IgG2b Kappa |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 5D12-A12 |
Tested applications | ELISA, FC, ICC, IHC, IP, WB |
Reactivity | Human |
Isotype | IgG2b Kappa |
Immunogen | Full length human HSP27 |
Concentration | 1 mg/ml |
Dilution range | WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
Conjugation | Unconjugated |
MW | 27kDa |
Target | HSP27 |
Entrez | 3315 |
UniProt ID | P04792 |
NCBI | NP_001531.1 |
Storage | -20°C |
Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated |
Alternative names | 28kDa heat shock protein Antibody, CMT2F Antibody, Read more... |
Note | For research use only |
Application notes | 0.5 µg/ml of SMC-161 was sufficient for detection of HSP27 in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IHC-P, WB | |
Bovine, Canine, Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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