HSP27 Antibody / HSPB1

Catalog Number: orb750207

Catalog Number	orb750207
Category	Antibodies
Description	It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Clonality	Monoclonal
Species/Host	Mouse
Isotype	Mouse IgG1, kappa
Conjugation	Unconjugated
Reactivity	Human
Immunogen	Partially purified HSP27 protein from the human breast cancer cell line MCF-7 was used as the immunogen for this HSP27 antibody.
UniProt ID	P04792
Tested applications	FACS, IF, IHC-P, WB
Dilution range	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application notes	Optimal dilution of the antibody should be determined by the researcher.
Antibody Type	Primary Antibody
Clone Number	SPM252
Formula	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard Information	This HSP27 antibody is available for research use only.
Note	For research use only

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone SPM252).

Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP27 antibody (clone SPM252, green) and Reddot nuclear stain (red).

Flow cytometry testing of PFA-fixed human MCF7 cells with HSP27 antibody (clone SPM252); Red=isotype control, Blue= HSP27 antibody.

Western blot testing of human HeLa cell lysate with HSP27 antibody (clone SPM252). Expected molecular weight: 24-29 kDa.

SDS-PAGE analysis of purified, BSA-free HSP27 antibody (clone SPM252) as confirmation of integrity and purity.