HSP27 Antibody

SKU: orb750207

Description

It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Research Area

Epigenetics, Infectious Diseases, Neuroscience

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The optimal dilution of the HSP27 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: SPM252
• Immunogen: Partially purified HSP27 protein from the human breast cancer cell line MCF-7 was used as the immunogen for this HSP27 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone SPM252).

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone SPM252).

Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP27 antibody (clone SPM252, green) and Reddot nuclear stain (red).

Flow cytometry testing of PFA-fixed human MCF7 cells with HSP27 antibody (clone SPM252); Red=isotype control, Blue= HSP27 antibody.

Western blot testing of human HeLa cell lysate with HSP27 antibody (clone SPM252). Expected molecular weight: 24-29 kDa.

SDS-PAGE analysis of purified, BSA-free HSP27 antibody (clone SPM252) as confirmation of integrity and purity.

Quick Database Links

UniProt

P04792