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Catalog Number | orb1256006 |
---|---|
Category | Antibodies |
Description | HSD11B2 Antibody |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 266-405 of human HSD11B2 (NP_000187.3). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 44kDa |
Target | HSD11B2 |
UniProt ID | P80365 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | HSD11B2, AME, AME1, HSD11K, HSD2, SDR9C3 Read more... |
Note | For research use only |
Application notes | WB: 1:200 - 1:1000IHC: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of HepG2 cells, using HSD11B2 antibody (orb1256006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human liver damage using HSD11B2 antibody (orb1256006) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HSD11B2 antibody (orb1256006) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using HSD11B2 antibody (orb1256006) at dilution of 1:100 (40x lens).
ELISA, FC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Equine, Guinea pig, Mouse, Rabbit, Sheep | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Canine, Equine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |