HSD11B2 Antibody

• Catalog Number: orb1256006
• Category: Antibodies
• Description: HSD11B2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 266-405 of human HSD11B2 (NP_000187.3).
• Concentration: batch dependent
• Dilution range: WB: 1:200 - 1:1000IHC: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 44kDa
• Target: HSD11B2
• UniProt ID: P80365
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: HSD11B2, AME, AME1, HSD11K, HSD2, SDR9C3
• Note: For research use only
• Application notes: WB: 1:200 - 1:1000IHC: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of HepG2 cells, using HSD11B2 antibody (orb1256006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded human liver damage using HSD11B2 antibody (orb1256006) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using HSD11B2 antibody (orb1256006) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using HSD11B2 antibody (orb1256006) at dilution of 1:100 (40x lens).