HSD11B2 Antibody

SKU: orb1256006

Description

HSD11B2 Antibody

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Rat
• Application Notes:
  WB: 1:200 - 1:1000IHC: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 266-405 of human HSD11B2 (NP_000187.3).
• Target: HSD11B2
• Molecular Weight: Observed: 44kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSD11B2, AME, AME1, HSD11K, HSD2, SDR9C3

Western blot analysis of extracts of HepG2 cells, using HSD11B2 antibody (orb1256006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded human liver damage using HSD11B2 antibody (orb1256006) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using HSD11B2 antibody (orb1256006) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using HSD11B2 antibody (orb1256006) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

HSD11B2

UniProt

P80365