HSD11B2 Rabbit Polyclonal Antibody

• Catalog Number: orb5473
• Category: Antibodies
• Description: HSD11B2 Rabbit Polyclonal Antibody
• Target: HSD11B2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Equine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: Recombinant human HSD11B2 protein
• UniProt ID: P80365
• RRID: AB_10934812
• MW: 45 kDa
• Tested applications: ELISA, WB
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Corticosteroid 11 beta dehydrogenase isozyme 2; 11
• Research Area: Cardiovascular Research, Cell Biology, Hormone Res
• Note: For research use only

A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HSD11B2) polyclonal Antibody, Unconjugated (orb5473) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-HSD11B2 (orb5473) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 48 kD.

Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Rat Stomach tissue lysates, Primary: Anti-HSD11B2 (orb5473) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kDa, Observed band size: 45 kDa.

Sample: Lane 1: Mouse Stomach tissue lysates, Lane 2: Rat Stomach tissue lysates, Primary: Anti-HSD11B2 (orb5473) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kDa, Observed band size: 45 kDa.