HSD11B2 Rabbit Polyclonal Antibody

SKU: orb5473

Description

HSD11B2 Rabbit Polyclonal Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB=1:500-2000, ELISA=1:5000-10000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Equine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human HSD11B2 protein
• Target: HSD11B2
• Molecular Weight: 45 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AME; AME1; HSD11K; HSD2; SDR9C3; 11HSD2; DHI2_BOVIN; HSD11B2; 11-DH2; 11-beta-HSD2; Corticosteroid 11-beta-dehydrogenase isozyme 2; NAD-dependent 11-beta-hydroxysteroid dehydrogenase; 1.1.1.-; DHI2_HUMAN; 11-beta-hydroxysteroid dehydrogenase type II (11-HSD type II | 11-beta-HSD type II); Short chain dehydrogenase/reductase family 9C member 3; DHI2_MOUSE; DHI2_RABIT; 11beta-OHSD type 2; DHI2_RAT; DHI2_SHEEP;

A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HSD11B2) polyclonal Antibody, Unconjugated (orb5473) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-HSD11B2 (orb5473) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 48 kD.

Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Rat Stomach tissue lysates, Primary: Anti-HSD11B2 (orb5473) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kDa, Observed band size: 45 kDa.

Sample: Lane 1: Mouse Stomach tissue lysates, Lane 2: Rat Stomach tissue lysates, Primary: Anti-HSD11B2 (orb5473) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kDa, Observed band size: 45 kDa.

Quick Database Links

Gene Symbol

HSD11B2

UniProt

P80365