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HPV-16 L1 Antibody

Catalog Number: orb606482

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb606482
CategoryAntibodies
DescriptionReacts with a protein of 57kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). It is the major capsid protein of HPV-16. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.
Species/HostMouse
ClonalityRecombinant
Clone NumberrHPV16L1/1058
Tested applicationsIHC-P
ReactivityVirus
IsotypeMouse IgG1, kappa
ImmunogenHuman papilloma virus type 16, major capsid protein L1, was used as the immunogen for the recombinant HPV16 L1 antibody.
Antibody TypePrimary Antibody
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis recombinant HPV16 L1 antibody is available for research use only.
UniProt IDP03101
StorageStore the recombinant HPV16 L1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the recombinant HPV16 L1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
HPV-16 L1 Antibody

IHC staining of FFPE human cervix with recombinant HPV16 L1 antibody (clone rHPV16L1/1058). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

HPV-16 L1 Antibody

SDS-PAGE analysis of purified, BSA-free recombinant HPV16 L1 antibody (clone rHPV16L1/1058) as confirmation of integrity and purity.

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