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HPV-16 L1 Antibody

Catalog Number: orb606483

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb606483
CategoryAntibodies
DescriptionReacts with a protein of 57kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). It is the major capsid protein of HPV-16. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.
ClonalityRecombinant
Species/HostRabbit
IsotypeRabbit IgG, kappa
ConjugationUnconjugated
ReactivityVirus
ImmunogenHuman papilloma virus type 16, major capsid protein L1, was used as the immunogen for the recombinant HPV16 L1 antibody.
UniProt IDP03101
Tested applicationsIHC-P
Dilution rangeImmunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application notesThe stated application concentrations are suggested starting points. Titration of the recombinant HPV16 L1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Antibody TypePrimary Antibody
Clone NumberHPV16/2058R
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis recombinant HPV16 L1 antibody is available for research use only.
NoteFor research use only
HPV-16 L1 Antibody

IHC staining of FFPE human cervix with recombinant HPV16 L1 antibody (clone HPV16/2058R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

HPV-16 L1 Antibody

SDS-PAGE analysis of purified, BSA-free recombinant HPV16 L1 antibody (clone HPV16/2058R) as confirmation of integrity and purity.

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    IHC-P

    Virus

    Mouse

    Recombinant

    Unconjugated

    20 μg, 100 μg
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    IF,  IHC-P

    Virus

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg
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    IHC

    Virus

    Mouse

    Monoclonal

    Unconjugated

    100 μg (without BSA and Azide), 100 μg, 20 μg
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    IF,  IHC-P

    Virus

    Monoclonal

    Unconjugated

    100 μg
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    IF,  IHC-P

    Mouse

    Monoclonal

    Unconjugated

    20 μg