HOXC6 Antibody

• Catalog Number: orb1248342
• Category: Antibodies
• Description: HOXC6 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The immunogen for this antibody is: C-PNVALNSTAYDPVRH
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Brain (Olfactory bulb) and in Rat and Pig Brain lysates (calculated MW of 26.9kDa according to NP_004494.1). In Mouse Brain lysates the band runs like a 27kDa band. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 26kDa
• Target: HOXC6
• UniProt ID: P09630
• NCBI: NP_004494.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: HOXC6, homeobox C6, CP25, HHO.C8, HOX3, HOX3C, hom
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Brain (Olfactory bulb) and in Rat and Pig Brain lysates (calculated MW of 26.9kDa according to NP_004494.1). In Mouse Brain lysates the band runs like a 27kDa band. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248342 (0.3 ug/ml) staining of Human Olfactory bulb lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248342 (0.01 ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248342 (0.03 ug/ml) staining of Pig Brain lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248342 (5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.