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Anti-HOXC6 Antibody

Catalog Number: orb256607

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb256607
CategoryAntibodies
DescriptionRabbit polyclonal antibody to HOXC6
TargetHOXC6
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Primate, Rat, Sheep
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HOXC6. The exact sequence is proprietary.
UniProt IDP10629, P09630
Tested applicationsIF, IH, WB
Dilution rangeWB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesanti HOX3C antibody, anti Homeobox protein Hox-C6
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NoteFor research use only
Entrez3223, 15425
Anti-HOXC6 Antibody

Western blot analysis of HOXC6 expression in A2780 (A), HEK293T (B), PC12 (C), 3T3L1 (D), PC3 (E) whole cell lysates. (Predicted band size: 26 kD; Observed band size: 27 kD)

Anti-HOXC6 Antibody

Immunohistochemical analysis of HOXC6 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-HOXC6 Antibody

Immunofluorescent analysis of HOXC6 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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