HOXA13 Polyclonal Antibody

• Catalog Number: orb1420841
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HOXA13.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Porcine, Rat
• UniProt ID: P31271
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Homeobox protein Hox-A13, Homeobox protein Hox-1J,
• Note: For research use only

Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-HOXA13 Polyclonal Antibody, Unconjugated 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug. Lane 2: Uterus (Mouse) Lysate at 40 ug. Lane 3: Liver (Rat) Lysate at 40 ug. Lane 4: Uterus (Rat) Lysate at 40 ug. Lane 5: 293T (Human) Cell Lysate at 30 ug. Lane 6: Hela (Human) Cell Lysate at 30 ug. Lane 7: U2os (Human) Cell Lysate at 30 ug. Primary: Anti-HOXA13 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 40 kD. Observed band size: 46 kD.