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HOXA13 Polyclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Reactivity | Porcine, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−HOXA13 Rabbit Polyclonal Antibody [orb157562]
IF, IHC-Fr, IHC-P, WB
Bovine, Equine, Gallus, Porcine, Rabbit, Sheep
Human, Mouse, Rat
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Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-HOXA13 Polyclonal Antibody, Unconjugated 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug. Lane 2: Uterus (Mouse) Lysate at 40 ug. Lane 3: Liver (Rat) Lysate at 40 ug. Lane 4: Uterus (Rat) Lysate at 40 ug. Lane 5: 293T (Human) Cell Lysate at 30 ug. Lane 6: Hela (Human) Cell Lysate at 30 ug. Lane 7: U2os (Human) Cell Lysate at 30 ug. Primary: Anti-HOXA13 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 40 kD. Observed band size: 46 kD.
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Protocol Information
HOXA13 Polyclonal Antibody (orb1420841)
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