HOXA13 Rabbit Polyclonal Antibody

• Catalog Number: orb157562
• Category: Antibodies
• Description: HOXA13 Rabbit Polyclonal Antibody
• Target: HOXA13
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Equine, Gallus, Porcine, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from Human HOXA13 (332-388/388aa)
• UniProt ID: P31271
• MW: 40 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Homeo box 1J; Homeo box A13; Homeobox 1J; Homeobox
• Note: For research use only

Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug, Lane 2: Uterus (Mouse) Lysate at 40 ug, Lane 3: Liver (Rat) Lysate at 40 ug, Lane 4: Uterus (Rat) Lysate at 40 ug, Lane 5: 293T (Human) Cell Lysate at 30 ug, Lane 6: Hela (Human) Cell Lysate at 30 ug, Lane 7: U2os (Human) Cell Lysate at 30 ug, Primary: Anti-HOXA13 (orb157562) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kD, Observed band size: 46 kD.

Tissue/Cell: human placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-HOXA13 Polyclonal Antibody, Unconjugated (orb157562) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.