HO-1 Antibody

SKU: orb1822444

Description

Rabbit Anti-Human HO-1 Polyclonal

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Epigenetics & Chromatin, Hematology, Immunology & Inflammation, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Reactivity: Canine, Human, Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of HO-1 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Human heme-oxygenase (HO-1) synthetic multiple antigenic peptide
• Target: HO-1
• Molecular Weight: 32kDa
• Purification: Protein A Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

HSP32, HMOX1, Heme oxygenase 1, HO, HO1, 32 kD, bK286B10, D8Wsu38e, heat shock protein 32 kD, heat shock protein 32kD, Heat shock protein, Heme oxygenase (decycling) 1, Hemox, HMOX 1, Hmox, Hmox1, HMOX1_HUMAN, HO 1, HO-1, Heme Oxygenase-1, Haem Oxygenase-1, Heat Shock Protein 32, Heme Oxygenase (Decyclizing), EC 1.14.14.18

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HO-1 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 100x. Heat Shocked at 42°C for 1h.

Western blot analysis of Human Cell line lysates showing detection of HO-1 protein using Rabbit Anti-HO-1 Polyclonal Antibody. Load: 15 μgprotein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HO-1 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HO-1 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HO-1 Polyclonal Antibody at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HO-1 Antibody. (C) Composite.

Quick Database Links

Gene Symbol

HO-1

UniProt

P09601

NCBI Gene ID

3162

RefSeq (Protein):NP_002124.1