HNRPAB Antibody (N-term)

• Catalog Number: orb1930465
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB20809
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 36225 Da
• Target: This HNRPAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HNRPAB.
• UniProt ID: Q99729
• NCBI: NP_112556.2, NP_004490.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Heterogeneous nuclear ribonucleoprotein A/B, hnRNP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of MCF-7 cell stained with HNRPAB Antibody (N-term).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRPAB primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HNRPAB immunoreactivity is localized to Nucleus significantly.

HNRPAB Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HNRPAB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

HNRPAB Antibody (N-term) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the HNRPAB antibody detected the HNRPAB protein (arrow).