HNRPAB Antibody (N-term)

SKU: orb1787865

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This HNRPAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HNRPAB. Antigen Region: 1-30 aa.
• Target: HNRNPAB
• Molecular Weight: 36225 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Heterogeneous nuclear ribonucleoprotein A/B, hnRNP A/B, APOBEC1-binding protein 1, ABBP-1, HNRNPAB, ABBP1, HNRPAB

HNRPAB Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HNRPAB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-HNRPAB Antibody (N-term) at 1:1000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: T47D whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of MCF-7 cell stained with HNRPAB Antibody (N-term).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRPAB primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HNRPAB immunoreactivity is localized to Nucleus significantly.

Quick Database Links

Gene Symbol

HNRNPAB

UniProt

Q99729

RefSeq (Protein):NP_112556.2, NP_004490.2