HNRNPD Antibody

• Catalog Number: orb1259391
• Category: Antibodies
• Description: HNRNPD Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of human HNRNPD (NP_002129.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 37-45kDa
• Target: HNRNPD
• UniProt ID: Q14103
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: HNRPD, P37, AUF1, AUF1A, HNRPD, hnRNPD0
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HNRNPD antibody (orb1259391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat liver using HNRNPD antibody (orb1259391) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using HNRNPD antibody (orb1259391) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using HNRNPD antibody (orb1259391) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using HNRNPD antibody (orb1259391) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using HNRNPD antibody (orb1259391) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using HNRNPD antibody (orb1259391) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using HNRNPD antibody (orb1259391) at dilution of 1:100. Blue: DAPI for nuclear staining.