HNRNPD Rabbit pAb Discontinued

SKU: orb539849

Description

Rabbit polyclonal antibody to HNRNPD

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, IP, WB
• Dilution Range: WB: 1:500-1:2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of human HNRNPD (NP_002129.2).
• Molecular Weight: 37-45kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HNRNPD, AUF1, AUF1A, HNRPD, P37, hnRNPD0

Immunofluorescence analysis of C6 cells using HNRNPD Rabbit pAb (orb539849) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using HNRNPD Rabbit pAb (orb539849) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using HNRNPD Rabbit pAb (orb539849) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using HNRNPD Rabbit pAb (orb539849) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded human lung cancer using HNRNPD Rabbit pAb (orb539849) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse brain using HNRNPD Rabbit pAb (orb539849) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat liver using HNRNPD Rabbit pAb (orb539849) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunoprecipitation analysis of 300 µg extracts of HeLa cells using 3 µg HNRNPD antibody (orb539849). Western blot was performed from the immunoprecipitate using HNRNPD antibody (orb539849) at a dilution of 1:1000.

Western blot analysis of various lysates using HNRNPD Rabbit pAb (orb539849) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Quick Database Links

UniProt

Q14103

Kailiang Qiao, Jingxia Han, Haohao Zhang, Yinan Li, Xiaohui Hou, Yan Jia, Yujie Sun, Huan Wang, Zheng Xu, Haoyang Liu, Heng Zhang, Huijuan Liu, Wei Zhang,Tao Sun Intratumor Mycoplasma promotes the initiation and progression of hepatocellular carcinoma Cell Rep, 42(12), 113563 (2023)

PubMed: 38088929

Applications

IHC

Reactivity

Mouse