HNRNPA0 Antibody

• Catalog Number: orb1257220
• Category: Antibodies
• Description: HNRNPA0 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HNRNPA0 (NP_006796.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 37kDa
• Target: HNRNPA0
• UniProt ID: Q13151
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: HNRPA0, HNRPA0
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HNRNPA0 antibody (orb1257220) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human esophagus using HNRNPA0 antibody (orb1257220) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of L-929 cells using HNRNPA0 Polyclonal Antibody (orb1257220) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of 293T cells, using 3 ug HNRNPA0 antibody (orb1257220). Western blot was performed from the immunoprecipitate using HNRNPA0 antibody (orb1257220) at a dilution of 1:1000.