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Description
Images & Validation
−
| Tested Applications | ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as a guideline try; IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB: 1/2000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). Flow Cyt: Use 1µg for 106 cells. |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 4D11 |
| Immunogen | Human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose |
| Target | hnRNP L |
| Purification | Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody (from supernatant) containing PBS. Does not contain sodium azide or rAlbumin |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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UniProt Details
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NCBI Gene Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
hnRNP L Monoclonal Antibody (orb1821316)
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