HNF4A Antibody (Center)

• Catalog Number: orb1931464
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB17745
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 52785 Da
• Target: This HNF4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-312 amino acids from the Central region of human HNF4A.
• UniProt ID: P41235
• NCBI: NP_001025175.1, NP_849181.1, NP_787110.2, NP_001025174.1, NP_001245284.1, NP_000448.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

HNF4A Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of HNF4A Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

HNF4A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of HNF4A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

HNF4A Antibody (Center) western blot analysis in 293, HepG2 cell line and rat liver tissue lysates (35 ug/lane). This demonstrates the HNF4A antibody detected the HNF4A protein (arrow).