HNF4A Antibody (Center)

SKU: orb1931464

Description

HNF4A Antibody (Center)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50, FC - 1:10-50
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 52785 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HNF4, NR2A1, TCF14

HNF4A Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of HNF4A Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

HNF4A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of HNF4A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

HNF4A Antibody (Center) western blot analysis in 293, HepG2 cell line and rat liver tissue lysates (35 ug/lane). This demonstrates the HNF4A antibody detected the HNF4A protein (arrow).

Quick Database Links

UniProt

P41235