Anti-HNF4 alpha/gamma Antibody

• Catalog Number: orb515416
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HNF4A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HNF4 alpha/gamma. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000, IHC-P: 1:50-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HNF4A; HNF4G
• Entrez: 3174, 30942, 3172, 15378
• UniProt ID: Q14541, P41235, P49698, Q9WUU6
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: HNF4A; HNF4; NR2A1; TCF14; Hepatocyte nuclear fact
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HNF4 alpha/gamma expression in HCT116 (A), CT26 (B), A549 (C) whole cell lysates. (Predicted band size: 52; 45 kD; Observed band size: 52 kD)

Immunohistochemical analysis of HNF4 alpha/gamma staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.