HLA-F Antibody

SKU: orb1925353

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:2000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,κ
• Clone No.: B4232EV621X44X32
• Immunogen: This HLA-F antibody is generated from a mouse immunized with a recombinate protein from the human region of human HLA-F.
• Target: HLAF
• Molecular Weight: 39062 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HLA class I histocompatibility antigen, alpha chain F, CDA12, HLA F antigen, Leukocyte antigen F, MHC class I antigen F, HLA-F, HLA-5.4, HLAF

Anti-HLA-F Antibody at 1:2000 dilution + Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-HLA-F Antibody at 1:1000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

HLAF

UniProt

P30511