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Catalog Number | orb545717 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against HLA-F conjugated to PE |
Clonality | Monoclonal |
Clone Number | 3D11 |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | Inclusion body-derived HLA-F heavy chain |
Antibody Type | Primary Antibody |
Concentration | 0.1 mg/ml |
Dilution range | Flow cytometry: Recommended dilution: 1-5 μg/ml. |
Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | PE |
Target | HLA-F |
Entrez | 3134 |
UniProt ID | P30511 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-CDA12 antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 1-5 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry multicolor intracellular staining pattern of human lymphocytes stained using anti-human CD20 (2H7) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/ml, red) or mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as anti-HLA-F PE concentration, black).
Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD20 (2H7) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/ml, red) or mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as anti-HLA-F PE concentration, black).
Separation of lymphocytes stained using anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/ml, red-filled) from lymphocytes stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as anti-HLA-F PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of human stimulated (PMA + Ionomycin) whole blood cells.
Separation of lymphocytes stained using anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/ml, red-filled) from lymphocytes stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as anti-HLA-F PE concentration, black-dashed) in flow cytometry analysis (surface staining) of human stimulated (PMA + Ionomycin) whole blood cells.