HLA-B Antibody (N-term)

• Catalog Number: orb1788367
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: 25-107 aa
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: H=40
• Target: This HLA-B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-107 amino acids from the N-terminal region of human HLA-B.
• UniProt ID: P30464
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: HLA class I histocompatibility antigen, B-15 alpha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded R. spleen section using HLA-B Antibody (N-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. spleen section using HLA-B Antibody (N-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. tonsil section using HLA-B Antibody (N-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Anti-HLA-B Antibody (N-term) at 1:2000 dilution + Human spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from Jurkat, Ramos cell line, human spleen, rat spleen tissue lysate (from left to right), using HLA-B Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.