HLA-ABC Antibody (MHC I)

SKU: orb2638534

Description

Reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.

Images & Validation

• Tested Applications: FACS, IF, WB
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  Optimal dilution of the HLA-ABC antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: 246-B8.E7
• Immunogen: Human peripheral blood leukocytes were used as the immunogen for the HLA-ABC antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of ThP-1 cell lysate with HLA-ABC antibody. Expected molecular weight of A/B/C: 40-41 kDa.

SDS-PAGE Analysis of Purified, BSA-Free HLA-ABC Antibody (clone 246-B8.E7). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P30443, P01889, P30499