HK3 Antibody

SKU: orb1255845

Description

HK3 Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HK3 (NP_002106.2).
• Target: HK3
• Molecular Weight: Observed: 110kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HK3, HKIII, Hexokinase 3, Hexokinase type III, HXK3, Hexokinase 3 (white cell), Hexokinase-3, HK III

Western blot analysis of extracts of various cell lines, using HK3 antibody (orb1255845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded mouse kidney using HK3 antibody (orb1255845) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using HK3 antibody (orb1255845) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

HK3

UniProt

P52790