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Description
Research Area
Epigenetics & Chromatin
Images & Validation
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Item 1 of 4
| Tested Applications | ChIP, DOT, ELISA, ICC, IHC-Fr, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as a guideline try up to 1:1000 for western blot. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).Can be blocked with Histone H3 peptide - tri methyl K9 |
| Reactivity | Drosophila, Hamster, Human, Porcine, Primate, Rabbit, Rat, Yeast |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 6F12-H4 |
| Immunogen | Synthetic peptide of Human Histone H3, tri methylated at K9 |
| Target | Histone H3 (tri methyl K9) |
| Purification | Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody (from supernatant) containing PBS + 0.1% sodium azide |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Histone H3.1 antibody;FLJ92264 antibody;H3 histone antibody;H3 histone family, member A antibody;H3/a antibody;H3/b antibody;H3/c antibody;H3/d antibody;h3/f antibody;H3/h antibody;H3/i antibody;H3/j antibody;H3/k antibody;H3/l antibody;H31_HUMAN antibody;H3F1K antibody;H3F3 antibody;H3FA antibody;H3FB antibody;H3FC antibody;H3FD antibody;H3FF antibody;H3FH antibody;H3FI antibody;H3FJ antibody;H3FK antibody;H3FL antibody;HIST1H3A antibody;HIST1H3B antibody;HIST1H3C antibody;HIST1H3D antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-Fr
Immunohistochemistry Frozen
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
ChIP
Chromatin Immunoprecipitation
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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Usually dispatched within 5-10 working days