HIST2H3C Antibody

SKU: orb344707

Description

Histone H3 K4me1 antibody

Images & Validation

• Tested Applications: ChIP, DOT, ELISA, IF, IHC, IP, WB
• Dilution Range: ELISA: 1:3,000 - 1:10,000, ChIP: 2-5µg/million cells, IHC: 2 - 5 µg/ml, IF: 2 - 5 µg/ml, IP: 2 mg/ml - 5 µg/ml, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  Anti-Histone H3 [Monomethyl Lys4] antibody has been tested in ELISA, Dot blot, and Western Blot. Histone3 K4me1 is useful for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Histone H3 K4me1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 4 of human Histone H3.2.
• Target: HIST2H3C
• Purity: Anti-Histone H3 K4me1 Antibody was purified from monospecific antiserum by protein A affinity purification. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Anti-Histone H3 Monomethyl Lys4 antibody, rabbit anti-Histone H3 K4 me1 antibody, H3 K4me1, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K4me1

Western Blot of Rabbit anti-Histone H3 K4Me1 antibody. Lane 1: Raji Whole Cell Lysate (p/n orb348672). Load: 35 µg per lane. Primary antibody: H3K4me1 antibody at 1:2000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15kDa for Histone H3.

Quick Database Links

Gene Symbol

HIST2H3C

UniProt

Q71DI3

RefSeq (Protein):NP_001005464.1