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Histone H3 K37me3 antibody

Histone H3 K37me3 antibody

Catalog Number: orb420419

DispatchUsually dispatched within 5-10 working days
$ 815.00
Catalog Numberorb420419
CategoryAntibodies
DescriptionHistone H3 K37me3 antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsDOT, IF, IHC, Multiplex Assay, WB
ReactivityC. elegans, Human
IsotypeIgG
ImmunogenHistone H3 [Trimethyl Lys37] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 37 of human Histone H3.
Concentration1.1 mg/mL
Dilution rangeIHC: 1:500, IF: 1:500, WB: 1µg/mL
Form/AppearanceLiquid (sterile filtered)
PurityAnti-Histone H3 [Trimethyl Lys37] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
ConjugationUnconjugated
UniProt IDQ71DI3
NCBINP_001005464
StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesrabbit anti-Histone H3 trimethyl Lys37 antibody, H
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NoteFor research use only
Application notesAnti-Histone H3 [Trimethyl Lys37] antibody is tested for Western Blot, Immunocytochemistry/Immunofluorescence, and Dot Blot. This antibody is useful in Chromatin Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.
Expiration Date12 months from date of receipt.
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