HIST2H3C Antibody

SKU: orb420417

Description

Histone H3 K37me1 antibody

Images & Validation

• Tested Applications: DOT, IF, Multiplex Assay, WB
• Dilution Range: WB: 1µg/mL
• Reactivity: C. elegans, Human, Mouse
• Application Notes:
  Anti-Histone H3 [Monomethyl Lys37] antibody is tested for Bot Blot, Western Blot, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Anti-Histone H3 is also useful for Dot Blot. Epi-Plus antibody production in collaboration with Novus Biologicals.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Histone H3 [Monomethyl Lys37] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic methylated peptide surrounding Lysine 37 of human Histone H3.
• Target: HIST2H3C
• Purity: Anti-Histone H3 [Monomethyl Lys37] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Histone H3 monomethyl Lys37 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87782, MGC87783, H3K37me1

Dot Blot of Rabbit Histone H3 [Monomethyl Lys37] Antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Monomethyl Lys37] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

Dot Blot of Rabbit Histone H3 [Monomethyl Lys37] Antibody. Lane 1: K37me1. Lane 2: K36me1. Lane 3: K36me2. Lane 4: K36me3. Lane 5: K36ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [Monomethyl Lys37] antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

Immunofluorescence of Anti-Histone H3 K37 me1: Histone H3 monomethyl Lys37 antibody was tested at 1:200 in HeLa cells with FITC (green). Cells and nuclei were counterstained with DAPI (blue) and DyLight 550 (red). (40X)

Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys37] Antibody. Lane 1: HeLa histone prep. Lane 2: NIH-3T3 histone prep. Lane 3: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Methyl Lys37] at 1 µg/ml for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.

Quick Database Links

Gene Symbol

HIST2H3C

UniProt

Q71DI3

RefSeq (Protein):NP_001005464