Histone H3 K27me3 antibody

• Catalog Number: orb420432
• Category: Antibodies
• Description: Histone H3 K27me3 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Histone H3 [Trimethyl Lys27] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27me3 site of human Histone H3.
• Concentration: 0.93 mg/ml
• Dilution range: ELISA: 1: 10,000, IF: 1:100, IP: 1:100, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Histone H3 [Trimethyl Lys27] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [Lys27 me3] from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q71DI3
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Histone H3 trimethyl Lys27 antibody, H
• Note: For research use only
• Application notes: Anti-Histone H3 [Trimethyl Lys27] antibody is tested for Dot Blot, IF, and Western Blot. This antibody is useful for ELISA and immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4kDa corresponding to the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.
• Expiration Date: 12 months from date of receipt.