You have no items in your shopping cart.
HIST3H3 Antibody
SKU: orb1258196
Featured
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 6
| Tested Applications | ChIP, IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Other, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human HIST3H3 (NP_003484.1). |
| Target | HIST3H3 |
| Molecular Weight | Observed: 17kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−H3F3A, H3t, H3.4, H3/g, H3FT
Similar Products
−- Item 1 of 48
Histone H3 Rabbit Polyclonal Antibody (Nuclear Loading Control) [orb10805]
FC, IF, IHC-Fr, IHC-P, WB
Bovine, Drosophila, Porcine, Rabbit
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
1 ml, 100 μl, 500 μl, 200 μl - Item 1 of 20
Histone H3 (tri methyl K79) Mouse Monoclonal Antibody [orb500508]
IF, IHC-Fr, IHC-P, WB
Mouse, Rat
Human, Mouse, Rat
Mouse
Monoclonal
Unconjugated
200 μg, 100 μl, 200 μl, 50 μl - Item 1 of 19
Histone H3 (di methyl K36) Mouse Monoclonal Antibody [orb500366]
IF, IHC-Fr, IHC-P
Mouse, Rat
Human, Mouse, Rat
Mouse
Monoclonal
Unconjugated
200 μg, 100 μl, 50 μl, 200 μl - Item 1 of 17
Histone H3 (Tri Methyl K4) Rabbit Polyclonal Antibody [orb1845]
ELISA, IF, IHC-Fr, IHC-P, WB
Mouse, Rat
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl - Item 1 of 14
Histone H3 Mouse Monoclonal Antibody (Nuclear Loading Control) [orb500825]
IF, IHC-Fr, IHC-P, WB
Hamster, Insect
Human, Mouse, Rat
Mouse
Monoclonal
Unconjugated
1 ml, 100 μl, 500 μl, 200 μl
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Western blot analysis of extracts of various cell lines, using HIST3H3 antibody (orb1258196) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human placenta using Histone H3 antibody (orb1258196) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Histone H3 antibody (orb1258196) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Histone H3 antibody (orb1258196) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HIST3H3 antibody (orb1258196) at dilution of 1:100. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using HIST3H3 antibody (orb1258196) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Quick Database Links
Gene Symbol
HIST3H3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ChIP
Chromatin Immunoprecipitation
HIST3H3 Antibody (orb1258196)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days