Histone H3 Antibody

• Catalog Number: orb1258196
• Category: Antibodies
• Description: Histone H3 Antibody
• Target: HIST3H3
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Other, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human HIST3H3 (NP_003484.1).
• UniProt ID: Q16695
• MW: Observed: 17kDa
• Tested applications: ChIP, IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200ChIP: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3F3A, H3t, H3.4, H3/g, H3FT
• Note: For research use only

Western blot analysis of extracts of various cell lines, using HIST3H3 antibody (orb1258196) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human placenta using Histone H3 antibody (orb1258196) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using Histone H3 antibody (orb1258196) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using Histone H3 antibody (orb1258196) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using HIST3H3 antibody (orb1258196) at dilution of 1:100. Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using HIST3H3 antibody (orb1258196) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.