HIST3H3 Antibody

SKU: orb1258196

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Description

HIST3H3 Antibody

Research Area

Signal Transduction

Images & Validation

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Item 1 of 6

Tested Applications	ChIP, IF, IHC, WB
Reactivity	Human, Mouse, Other, Rat
Application Notes	WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200ChIP: 1:50 - 1:200

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human HIST3H3 (NP_003484.1).
Target	HIST3H3
Molecular Weight	Observed: 17kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentration	batch dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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H3F3A, H3t, H3.4, H3/g, H3FT

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Western blot analysis of extracts of various cell lines, using HIST3H3 antibody (orb1258196) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human placenta using Histone H3 antibody (orb1258196) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using Histone H3 antibody (orb1258196) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using Histone H3 antibody (orb1258196) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using HIST3H3 antibody (orb1258196) at dilution of 1:100. Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using HIST3H3 antibody (orb1258196) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Quick Database Links

Gene Symbol

HIST3H3

UniProt

Q16695

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ChIP

Chromatin Immunoprecipitation

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