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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Other, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human HIST3H3 (NP_003484.1). |
| Target | HIST3H3 |
| Molecular Weight | Observed: 17kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−H3F3A, H3t, H3.4, H3/g, H3FT
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Western blot analysis of extracts of various cell lines, using Histone H3 antibody (orb1258194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat kidney using Histone H3 antibody (orb1258194) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using Histone H3 antibody (orb1258194) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Histone H3 antibody (orb1258194) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 150 ug extracts of MCF7 cells using 3 ug Histone H3 antibody (orb1258194). Western blot was performed from the immunoprecipitate using Histone H3 antibody (orb1258194) at a dilution of 1:500.
Quick Database Links
Gene Symbol
HIST3H3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
HIST3H3 Antibody (orb1258194)
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