Histone H1 Antibody

SKU: orb385884

Description

Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. [UniProt]

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, IHC-P
• Dilution Range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human, Rat
• Application Notes:
  Optimal dilution of the Histone H1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: OSHT-2
• Immunogen: Recombinant full-length human protein was used as the immunogen for the Histone H1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human angiosarcoma and Histone H1 antibody (clone OSHT-2). Staining of formalin/paraffin tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human tonsil and Histone H1 antibody (clone OSHT-2). Staining of formalin/paraffin tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human pancreas and Histone H1 antibody (clone OSHT-2). Staining of formalin/paraffin tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE rat pancreas and Histone H1 antibody (clone OSHT-2). Staining of formalin/paraffin tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of permeabilized human HeLa cells with Histone H1 antibody (green, clone OSHT-2) and Phalloidin.

Quick Database Links

UniProt

P07305