Histone H1 Antibody

SKU: orb749552

Description

Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
• Reactivity: Human, Rat
• Application Notes:
  Optimal dilution of the Histone H1 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: HH1/957
• Immunogen: Recombinant full-length human protein was used as the immunogen for the Histone H1 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Histone H1 antibody (clone HH1/957).

IHC: Formalin-fixed, paraffin-embedded rat pancreas stained with Histone H1 antibody (clone HH1/957).

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with Histone H1 antibody (clone HH1/957).

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with Histone H1 antibody (clone HH1/957).

IHC: FFPE human testicular carcinoma tested with Histone H1 antibody (clone HH1/957).

IHC: FFPE human pancreas tested with Histone H1 antibody (clone HH1/957).

IHC: FFPE human angiosarcoma tested with Histone H1 antibody (clone HH1/957).

Immunofluorescent staining of permeabilized human HeLa cells with Histone H1 antibody (green, clone HH1/957) and Phalloidin.

Flow cytometry testing of permeabilized human HeLa cells with Histone H1 antibody (clone HH1/957); Red = isotype control, Blue = Histone H1 antibody.

Western blot testing of human heart lysate with Histone H1 antibody. Predicted molecular weight ~20 kDa but can be observed at 27-30 kDa.

Quick Database Links

UniProt

P07305