HRH1 Antibody

SKU: orb1247396

Description

HRH1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 55kDa and 37kDa in Human Duodenum lysate after 0.3µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the 37kDa band we observe given the calculated size of 55.8kDa according to NP_000852.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional band).Immunohistochemistry:In paraffin embedded Human Colon shows membranous staining. Recommended concentration: 4-6µg/mL. Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/mL. This product has been successfully used in IHC on Rat (PMID: 30226827).Immunofluorescence: This product has been successfully used in IF on Rat (PMID: 30413645).

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: CNENFKKTFKRILH
• Target: HRH1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HRH1, histamine receptor H1, HGNC:5182, H1-R, hisH1, histamine receptor, subclass H1, OTTHUMP00000160133, histamine H(1) receptor

Quick Database Links

Gene Symbol

HRH1

UniProt

P35367

RefSeq (Protein):NP_001091681.1, NP_001091683.1, NP_000852.1, NP_001091682.1