Histamine Receptor H1 Antibody

• Catalog Number: orb1247396
• Category: Antibodies
• Description: Histamine Receptor H1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC
• Predicted Reactivity: Bovine, Canine, Mouse
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: CNENFKKTFKRILH
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HRH1
• UniProt ID: P35367
• NCBI: NP_001091682.1, NP_001091683.1, NP_000852.1, NP_001091681.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: HRH1, histamine receptor H1, HGNC:5182, H1-R, hisH
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 55kDa and 37kDa in Human Duodenum lysate after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the 37kDa band we observe given the calculated size of 55.8kDa according to NP_000852.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional band).Immunohistochemistry:In paraffin embedded Human Colon shows membranous staining. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml. This product has been successfully used in IHC on Rat (PMID: 30226827).Immunofluorescence: This product has been successfully used in IF on Rat (PMID: 30413645).
• Expiration Date: 12 months from date of receipt.

orb1247396 (4 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1247396 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.