Goat anti-Histamine receptor H1(internal) Antibody

SKU: orb19456

Description

Goat polyclonal antibody to HRH1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.5-1.5 μg/ml, IHC-P: 2-4µg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Lung shows membranous staining of epitheilial cells in tertiar bronchus and in alveoli. Recommended concentration, 2-4µg/ml..WB: Approx 55kDa band observed in Human Heart lysates (calculated MW of 55.8kDa according to NP_000852.1). Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Histamine receptor H1(internal)
• Molecular Weight: 55.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti histamine receptor H1 antibody, anti H1-R antibody, anti HRH1 antibody, anti hisH1 antibody, anti histamine receptor, subclass H1 antibody

orb19456 (0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of human Heart lysate using HRH1 antibody

Immunohistochemical staining of human Lung using HRH1 antibody

Quick Database Links

Gene Symbol

Histamine receptor H1(internal)

NCBI Gene ID

3269