Goat anti-Histamine receptor H1(internal) Antibody

• Catalog Number: orb19456
• Category: Antibodies
• Description: Goat polyclonal antibody to HRH1
• Target: Histamine receptor H1(internal)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QQTSVRREDKCETD
• RRID: AB_10929410
• MW: 55.8
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.5-1.5 μg/ml, IHC-P: 2-4µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Lung shows membranous staining of epitheilial cells in tertiar bronchus and in alveoli. Recommended concentration, 2-4µg/ml..WB: Approx 55kDa band observed in Human Heart lysates (calculated MW of 55.8kDa according to NP_000852.1). Recommended concentration: 0.5-1.5 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti histamine receptor H1 antibody, anti H1-R ant
• Research Area: Epigenetics, GPCR, Neuroscience, Signaling Pathway
• Note: For research use only
• Entrez: 3269

orb19456 (0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of human Heart lysate using HRH1 antibody

Immunohistochemical staining of human Lung using HRH1 antibody