HRH1 Antibody

SKU: orb1247395

Description

HRH1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in Human Heart lysates (calculated MW of 55.8kDa according to NP_000852.1). Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:In paraffin embedded Human Lung shows membranous staining of epitheilial cells in tertiar bronchus and in alveoli. Recommended concentration, 2-4ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: QQTSVRREDKCETD
• Target: HRH1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

histamine receptor H1, H1-R, HRH1, hisH1 , histamine receptor, subclass H1

orb1247395 (0.5 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247395 (4 ug/ml) staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Quick Database Links

Gene Symbol

HRH1

UniProt

P35367

RefSeq (Protein):NP_000852.1