HIST1H3A (Ab-23) Antibody

• Catalog Number: orb517048
• Category: Antibodies
• Description: This HIST1H3A (Ab-23) Antibody is an unconjugated polyclonal product. It tagets HIST1H3A using a Peptide sequence around site of Lys (23) derived from Human Histone H3.1 as the immunogen. This antibody is suitable for ChIP, ELISA, IF, IHC, WB. Purification: Antigen Affinity Purified.
• Target: HIST1H3A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Purification: Antigen Affinity Purified
• Immunogen: Peptide sequence around site of Lys (23) derived from Human Histone H3.1
• UniProt ID: P68431
• Tested applications: ChIP, ELISA, IF, IHC, WB
• Dilution range: WB:1:500-1:5000, IHC-P:1:10-1:100, IF:1:50-1:200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3 histone family member E pseudogene antibody; H3
• Research Area: Epigenetics & Chromatin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- HIST1H3A (Ab-23) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time

Western blot aanalysis of Hela whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Rat liver tissueusing HIST1H3A (Ab-23) antibody

Immunofluorescence staining of A549 cells using HIST1H3A (Ab-23) antibody

Immunohistochemistry of paraffin-embedded human colon cancer tissue using HIST1H3A (Ab-23) antibody