His-tag rabbit pAb

• Catalog Number: orb763951
• Category: Antibodies
• Description: Rabbit polyclonal antibody to His-tag
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: All
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: 6X His synthetic peptide conjugated to KLH.
• MW: 15
• Tested applications: ELISA, WB
• Dilution range: Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-6 His epitope tag antibody, anti-Hexa His tag
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis using His-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing His tag was diluted at 1:20000.

Western Blot analysis of HIS-protein cells using His-tag Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000.