His Tag Antibody

SKU: orb1927577

Description

His Tag Antibody

Images & Validation

• Tested Applications: WB
• Dilution range: WB - 1：1000

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Western blot analysis of His Tag Antibody in his-tag TBB1 protein (35 ug/lane). His-tag TBB1 (arrow) was detected using the purified Pab.

All lanes: Anti-His Tag Antibody at 1:2000 dilution. Lane 1: 12-tag recombinant protein lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Observed band size: 50kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-His Tag Antibody at 1:2000 dilution. Lane 1: recombinant protein with His tag lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Observed band size: 50kDa. Blocking/Dilution buffer: 5% NFDM/TBST.