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HIRIP3 Antibody
SKU: orb1256659
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Description
Images & Validation
−Item 1 of 11
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 417-556 of human HIRIP3 (NP_003600.2). |
| Target | HIRIP3 |
| Molecular Weight | Observed: 62kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−HIRA-interacting protein 3, HIRIP3
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Western blot analysis of extracts of various cell lines, using HIRIP3 antibody (orb1256659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using HIRIP3 antibody (orb1256659) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using HIRIP3 antibody (orb1256659) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HIRIP3 antibody (orb1256659) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using HIRIP3 antibody (orb1256659) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using HIRIP3 antibody (orb1256659) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using HIRIP3 antibody (orb1256659) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using HIRIP3 antibody (orb1256659) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HIRIP3 antibody (orb1256659) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ZNF148 antibody (orb1256668) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HIRIP3 antibody (orb1256659).
Quick Database Links
Gene Symbol
HIRIP3
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
HIRIP3 Antibody (orb1256659)
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