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Catalog Number | orb412526 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to HIRIP3 |
Target | HIRIP3 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Immunogen | Recombinant full length protein of human HIRIP3 |
UniProt ID | Q8BLH7, Q9BW71 |
Tested applications | IF, IH, WB |
Dilution range | WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200 |
Antibody Type | Primary Antibody |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | anti-HIRA-interacting protein 3 antibody |
Note | For research use only |
Entrez | 8479, 233876 |
Western blot analysis of HIRIP3 expression in Hela (A), Jurkat (B), mouse liver (C), rat heart (D) whole cell lysates. (Predicted band size: 19; 27; 61 kD; Observed band size: 62 kD)
Immunofluorescent analysis of HIRIP3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.