HIF3 alpha Polyclonal Antibody

• Catalog Number: orb1417293
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HIF3 alpha.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Porcine, Rat
• Conjugation: Unconjugated
• UniProt ID: Q9Y2N7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Hypoxia-inducible factor 3-alpha, HIF-3-alpha, HIF
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lung (Mouse) Lysate at 40 ug. Primary: Anti-HIF3 alpha at 1/500 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 74 kD. Observed band size: 75 kD.

Blank control (blue line): A549 (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice).Primary Antibody (green line): Rabbit Anti-HIF3 alpha antibody, ilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg /test.

Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HIF3 alpha) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.