HIF3A Rabbit Polyclonal Antibody

• Catalog Number: orb577363
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HIF3A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Human
• Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HIF3A
• Concentration: 1.0 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 72 kDa
• Target: HIF3A
• UniProt ID: Q9Y2N7
• Protein Sequence: Synthetic peptide located within the following region: PGSLQDPSTPLLNLNEPLGLGPSLLSPYSDEDTTQPGGPFQPRAGSAQAD
• NCBI: NP_690007
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: IPAS, MOP7, PASD7, HIF-3A, bHLHe17, HIF3-alpha-1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. Multiple isoforms from 83 kDa to 65 kDa contain the immunizing peptide. Recommended dilution for this antibody is 1-3 ug/ml.

Human kidney

WB Suggested Anti-HIF3A Antibody Titration: 1.25 ug/ml, Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that HIF3A is expressed in HepG2.