HIF1AN Antibody

• Catalog Number: orb1257475
• Category: Antibodies
• Description: HIF1AN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human HIF1AN (NP_060372.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 40kDa
• Target: HIF1AN
• UniProt ID: Q9NWT6
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: HIF1AN, DKFZp762F1811, FIH1, FLJ20615, FLJ22027
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HIF1AN antibody (orb1257475) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat brain using HIF1AN antibody (orb1257475) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using HIF1AN antibody (orb1257475) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using HIF1AN antibody (orb1257475) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug HIF1AN antibody (orb1257475). Western blot was performed from the immunoprecipitate using HIF1AN antibody (orb1257475) at a dilution of 1:1000.