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HIF1AN Antibody
SKU: orb1257475
Featured
Description
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human HIF1AN (NP_060372.2). |
| Target | HIF1AN |
| Molecular Weight | Observed: 40kDa |
| Purification | Affinity purification |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−HIF1AN, DKFZp762F1811, FIH1, FLJ20615, FLJ22027
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Western blot analysis of extracts of various cell lines, using HIF1AN antibody (orb1257475) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using HIF1AN antibody (orb1257475) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HIF1AN antibody (orb1257475) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HIF1AN antibody (orb1257475) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug HIF1AN antibody (orb1257475). Western blot was performed from the immunoprecipitate using HIF1AN antibody (orb1257475) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
HIF1AN
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
HIF1AN Antibody (orb1257475)
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