HIF1AN Antibody (C-term)

• Catalog Number: orb1925539
• Category: Antibodies
• Description: HIF1AN Antibody (C-term)
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,k
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: Q9NWT6
• MW: 40285 Da
• Tested applications: IF, WB
• Dilution range: IF - 1:25, WB - 1:4000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: FIH1
• Research Area: Cancer Biology, Cardiovascular Research
• Note: For research use only

All lanes: Anti-HIF1AN Antibody (C-term) at 1:4000 dilution. Lane 1: 293 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: ZR-75-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling HIF1AN at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).