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HIF1 alpha Rabbit pAb

SKU: orb1563004

Description

HIF1 alpha Rabbit pAb

Research Area

Cardiovascular Research

Images & Validation

Tested ApplicationsELISA, ICC, IF, IHC-Fr, IHC-P, WB
Dilution RangeWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
ReactivityHuman, Mouse, Rat

Key Properties

Antibody TypePrimary Antibody
ClonalityPolyclonal
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human HIF-1alpha.
TargetHIF1A
Molecular WeightCalculated MW: 93 kDa; Observed MW: 92-130 kDa
PurificationAffinity Purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesLiquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS doma

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HIF1 alpha Rabbit pAb

Western blot analysis of HIF1 alpha in LOVO lysates using HIF1 alpha antibody.

HIF1 alpha Rabbit pAb

Immunohistochemistry analysis of paraffin-embedded mouse colon tissue using HIF1 alpha antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

HIF1 alpha Rabbit pAb

Immunohistochemistry analysis of paraffin-embedded rat lung tissue using HIF1 alpha antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

HIF1 alpha Rabbit pAb (orb1563004)

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Available Sizes

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20 μl
$ 120.00
50 μl
$ 210.00
100 μl
$ 350.00
DispatchUsually dispatched within 5-10 working days
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