HIF1Alpha Antibody (Center)

• Catalog Number: orb1928917
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB14548
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:250, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 92670 Da
• Target: This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 amino acids from the Central region of human HIF1Alpha.
• UniProt ID: Q16665
• NCBI: NP_001521.1, NP_851397.1, NP_001230013.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from Raji cell line, untreated or treated with CoCl2 (0. 1mM), using HIF1Alpha Antibody (Center) (upper) or Beta-actin (lower).

Western blot analysis of lysates from Raji cell line, untreated or treated with CoCl2 (0. 1mM), using HIF1Alpha Antibody (Center) (upper) or Beta-actin (lower).

HIF1Alpha Antibody (Center) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of HIF1Alpha Antibody (Center) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

HIF1Alpha Antibody (Center) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIF1Alpha Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.