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Description
Research Area
Cancer Biology
Images & Validation
−| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution Range | IF - 1:10-50, WB - 1:250, IHC-P - 1:50-100, FC - 1:10-50 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 amino acids from the Central region of human HIF1Alpha. Antigen Region: 519-547 aa. |
| Target | HIF1A {ECO:0000303|PubMed:7539918, ECO:0000312|HGNC:HGNC:4910} |
| Molecular Weight | 92670 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8

Quality Guarantee
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Quick Database Links
Gene Symbol
HIF1A {ECO:0000303|PubMed:7539918, ECO:0000312|HGNC:HGNC:4910}
UniProt
RefSeq (Protein):NP_001230013.1, NP_001521.1, NP_851397.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001230013.1, NP_001521.1, NP_851397.1 |
|---|
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence




