HIF1Alpha Antibody (Center)

SKU: orb1928917

Description

HIF1Alpha Antibody (Center)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: WB - 1:250
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 92670 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot analysis of lysates from Raji cell line, untreated or treated with CoCl2 (0. 1mM), using HIF1Alpha Antibody (Center) (upper) or Beta-actin (lower).

Western blot analysis of lysates from Raji cell line, untreated or treated with CoCl2 (0. 1mM), using HIF1Alpha Antibody (Center) (upper) or Beta-actin (lower).

HIF1Alpha Antibody (Center) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of HIF1Alpha Antibody (Center) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

HIF1Alpha Antibody (Center) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIF1Alpha Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

UniProt

Q16665