HIC1 Antibody

• Catalog Number: orb1248399
• Category: Antibodies
• Description: HIC1 Antibody
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: NTHCAELYASGP
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HIC1
• UniProt ID: Q14526
• NCBI: NP_001091672.1, NP_006488.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: HIC1, hypermethylated in cancer 1, ZBTB29, ZNF901,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kDa is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kDa according to NP_006488.2. Recommended concentration, 0.5-1ug/ml.
• Expiration Date: 12 months from date of receipt.

HEK293 overexpressing Human HIC1 and probed with orb1248399 (0.5 ug/ml) with the mock transfection in second lane.

orb1248399 (1 ug/ml) staining of A549 (A) and Jurkat (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.