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Catalog Number | orb1248399 |
---|---|
Category | Antibodies |
Description | HIC1 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Canine, Mouse, Porcine, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: NTHCAELYASGP |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kDa is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kDa according to NP_006488.2. Recommended concentration, 0.5-1ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | HIC1 |
UniProt ID | Q14526 |
NCBI | NP_001091672.1, NP_006488.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | HIC1, hypermethylated in cancer 1, ZBTB29, ZNF901, Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kDa is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kDa according to NP_006488.2. Recommended concentration, 0.5-1ug/ml. |
Expiration Date | 12 months from date of receipt. |
HEK293 overexpressing Human HIC1 and probed with orb1248399 (0.5 ug/ml) with the mock transfection in second lane.
orb1248399 (1 ug/ml) staining of A549 (A) and Jurkat (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA, WB | |
Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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