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Catalog Number | orb131697 |
---|---|
Category | Antibodies |
Description | Goat polyclonal to HIC1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:128000, WB: 2-3 μg/ml |
Conjugation | Unconjugated |
MW | 74.5; 76.5 |
Target | HIC1 (aa193-204) |
Entrez | 3090 |
Protein Sequence | NTHCAELYASGP |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | HIC1; hypermethylated in cancer 1; ZBTB29; ZNF901; Read more... |
Note | For research use only |
Application notes | In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kDa is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kDa according to NP_006488.2. Recommended concentration, 0.5-1 μg/ml.WB: In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kDa is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kDa according to NP_006488.2. Recommended concentration, 0.5-1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Human HIC1 using HIC1 antibody