HIC1 (aa193-204) Antibody

SKU: orb131697

Description

Goat polyclonal to HIC1

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:128000, WB: 2-3 μg/ml
• Reactivity: Mouse
• Predicted Reactivity: Canine, Human, Porcine, Rat
• Application Notes:
  In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kDa is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kDa according to NP_006488.2. Recommended concentration, 0.5-1 μg/ml.WB: In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kDa is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kDa according to NP_006488.2. Recommended concentration, 0.5-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: HIC1 (aa193-204)
• Protein Sequence: NTHCAELYASGP
• Molecular Weight: 74.5; 76.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HIC1; hypermethylated in cancer 1; ZBTB29; ZNF901; hic-1; hypermethylated in cancer 1 protein; zinc finger and BTB domain-containing protein 29

Western blot analysis of Human HIC1 using HIC1 antibody

Quick Database Links

Gene Symbol

HIC1 (aa193-204)

NCBI Gene ID

3090