HHLA2 Antibody

• Catalog Number: orb1270692
• Category: Antibodies
• Description: HHLA2 Antibody
• Target: HHLA2
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Immunogen: This HHLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human HHLA2.
• UniProt ID: Q9UM44
• MW: 47 kDa
• Tested applications: FC, IHC-P, WB
• Application notes: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HERV-H LTR-associating protein 2, Human endogenous
• Note: For research use only
• NCBI: Q9UM44

Western Blot at 1:2000 dilution Lane 1: human pancreas lysate Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane.

HHLA2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in MDA-MB453, CEM, HL-60, MDA-MB231 cell line lysates (35 ug/lane).